Designing reproducible PCR assays involves optimizing multiple moving targets, from standardizing each component in sometimes minute reaction volumes to planning ahead to ensure long-term and secure ...
Products are designed to resolve common PCR problems like primer dimer formation and mispriming. TATAA Biocenter will sell the line of CleanAmp™ Products produced by TriLink BioTechnologies for Hot ...
The key to an optimal PCR experiment is primer design. In general, primers should be between 18 and 24 nucleotides long and have a GC content between 40 and 60 percent. Ideally primers should contain ...
Indexes vary greatly and perform differently in different conditions. Some designs are predisposed to dimerization, while others are optimized at certain concentrations, and some will not be optimized ...