From Live Cells to Lysates: Adapting NanoBiT to a Biochemical Assay Format - Promega Connections
From Live Cells to Lysates: Adapting NanoBiT to a Biochemic…
Apr 16, 2020
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NanoBiT is a new technology for studying protein interactions that allows real-time analysis in live cells. The low-affinity protein complementation reporter is more sensitive than split firefly luciferase and binding is reversible, allowing detection of protein association and disassociation. Learn more>> http://bit.ly/2qGegEb | Genetic Engineering & Biotechnology News
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NanoBiT is a new technology for studying protein interactions that …
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How does NanoBiT compare to split firefly luciferase? The bright signal of the NanoBiT™ PPI Assay allows fusion proteins to be expressed at or near physiological levels in many cases, helping to reduce experimental artifacts. In this example, a FKBP:FRB model system is used to compare NanoBiT to a split firefly luciferase system. Both approaches gave similar EC50 values for rapamycin activation, but showed major differences in luminescence intensity. Learn more here: :http://bit.ly/1Sf3vyJ | Pro
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How does NanoBiT compare to split firefly luciferase? The bright signa…
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Promega NanoBiT Complementation Assay Named a Top 10 Innovation
Promega NanoBiT Complementation Assay Named a Top 10 Innovation
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