Double Staining of Intracellular Cytokine Proteins and T-lymphocyte Subsets. Evaluation of the Method in Blood and Bronchoalveolar Lavage Fluid, Journal of Molecular Histology | DeepDyve
deepdyve.com
Double Staining of Intracellular Cytokine Proteins and T-lymphocyte Subsets. Evaluation of the Method in Blood and Bronchoalveolar Lavage Fluid, Journal of Molecular
An immunocytochemical staining method has been developed for simultaneous staining of both cell surface markers (CD4 and CD8) and intracellular cytokine proteins IFN-γ, IL-4 and IL-5. Cell surface molecules were visualized with alkaline phosphatase, which was developed by Fast Blue BB. Intracellular cytokine proteins were detected by amino ...
Dec 16, 2014
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